Polymerase Chain Reaction
Biology ⇒ Biotechnology and Applications
Polymerase Chain Reaction starts at 11 and continues till grade 12.
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See sample questions for grade 11
Describe one limitation of PCR.
Describe the difference between conventional PCR and real-time (quantitative) PCR.
Describe the importance of the denaturation step in PCR.
Explain the significance of the annealing temperature in PCR.
A PCR reaction is set up with all components except primers. Will amplification occur? Yes or No.
A scientist wants to amplify a specific gene from a small DNA sample. Which technique should they use?
(1) Gel electrophoresis
(2) PCR
(3) Southern blotting
(4) DNA sequencing
If a PCR reaction is run for 10 cycles, starting with 2 DNA molecules, how many DNA molecules will be present at the end?
Context: A forensic scientist is analyzing a crime scene sample with a very low amount of degraded DNA. Which PCR modification would be most helpful in successfully amplifying the target DNA?
(1) Using nested PCR
(2) Increasing the extension temperature
(3) Decreasing the number of cycles
(4) Using shorter primers
A scientist wants to amplify a specific gene from a small DNA sample. Which technique should they use?
(1) Gel electrophoresis
(2) PCR
(3) Southern blotting
(4) DNA sequencing
At approximately what temperature does the denaturation step of PCR occur?
(1) 37°C
(2) 55°C
(3) 72°C
(4) 94°C
PCR is an example of which type of DNA replication?
(1) In vivo
(2) In vitro
(3) In situ
(4) In silico
Which of the following best describes the role of dNTPs in PCR?
(1) They act as primers
(2) They are the building blocks for new DNA strands
(3) They denature the DNA
(4) They act as enzymes
Fill in the blank: PCR requires two short single-stranded DNA sequences called ________.
Fill in the blank: The annealing step in PCR allows ________ to bind to the template DNA.
Fill in the blank: The original DNA sequence that is copied during PCR is called the ________.
Fill in the blank: The process of PCR involves repeated cycles of ________, annealing, and extension.
A PCR reaction is set up with all components except primers. Will amplification occur? Yes or No.
PCR can be used to amplify RNA directly. True or False?
True or False: PCR can be used to amplify a single DNA molecule to millions of copies.
True or False: PCR can be used to amplify both DNA and RNA directly.
