Polymerase Chain Reaction
Biology ⇒ Biotechnology and Applications
Polymerase Chain Reaction starts at 11 and continues till grade 12.
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See sample questions for grade 12
A PCR reaction contains 1,000 template DNA molecules. After 10 cycles, how many DNA molecules will there be, assuming 100% efficiency?
A PCR reaction is set up with 30 cycles. Ideally, how many copies of the target DNA will be produced from a single template molecule?
A student sets up a PCR reaction but forgets to add primers. What will happen?
Describe one application of PCR in the field of medicine.
A PCR reaction contains 1,000 template DNA molecules. After 10 cycles, how many DNA molecules will there be, assuming 100% efficiency?
A student sets up a PCR reaction but forgets to add primers. What will happen?
A laboratory technician accidentally uses a DNA polymerase that is not thermostable in a PCR reaction. Predict and explain the outcome of the PCR.
A researcher is attempting to amplify a DNA fragment with a high GC content using PCR. Explain two modifications to the standard PCR protocol that could improve the amplification of such GC-rich templates.
A scientist wants to amplify a specific gene from a sample containing a mixture of DNA. Which technique should they use? (1) Gel electrophoresis (2) PCR (3) Western blotting (4) Southern blotting
PCR is used to amplify which of the following? (1) Proteins (2) DNA (3) Lipids (4) Carbohydrates
Which component is NOT required for a standard PCR reaction? (1) DNA template (2) Primers (3) RNA polymerase (4) dNTPs
Which of the following is a common use of PCR in agriculture? (1) Identifying genetically modified organisms (2) Measuring soil pH (3) Increasing crop yield (4) Irrigation scheduling
Fill in the blank: The enzyme commonly used in PCR to synthesize new DNA strands is called _______.
Fill in the blank: The extension step in PCR is usually carried out at _______ degrees Celsius.
Fill in the blank: The first cycle of PCR produces _______ double-stranded DNA molecules from one double-stranded DNA template.
Fill in the blank: The number of DNA copies after n cycles of PCR is ideally _______ times the initial number of copies.
True or False: PCR can be automated using a thermal cycler.
True or False: PCR can be used in forensic science for DNA fingerprinting.
True or False: PCR can be used to amplify both nuclear and mitochondrial DNA.
True or False: PCR can be used to amplify DNA from ancient or degraded samples.
