Principles of Genetic Engineering
Biology ⇒ Biotechnology and Applications
Principles of Genetic Engineering starts at 9 and continues till grade 12.
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Describe the function of restriction enzymes in genetic engineering.
Describe the process of transformation in genetic engineering.
Explain the role of selectable markers in genetic engineering.
Explain why genetic engineering is important in medicine.
Explain why plasmids are commonly used as vectors in genetic engineering.
What is a plasmid?
What is meant by the term 'recombinant DNA'?
A scientist wants to insert a human gene into a bacterial plasmid. The plasmid has two restriction sites: EcoRI and HindIII. The human gene is flanked by EcoRI sites. Which restriction enzyme(s) should the scientist use to ensure successful insertion, and why?
Which of the following is a benefit of genetically modified crops?
(1) Increased resistance to pests
(2) Decreased nutritional value
(3) Reduced shelf life
(4) Lower yield
Which of the following is a commonly used vector in genetic engineering?
(1) Virus
(2) Plasmid
(3) Bacterium
(4) Fungi
Which of the following is a limitation of genetic engineering?
(1) Ethical concerns
(2) Increased crop yield
(3) Disease resistance
(4) Improved nutrition
Which of the following is a reason for using restriction enzymes in genetic engineering?
(1) To join DNA fragments
(2) To cut DNA at specific sites
(3) To replicate DNA
(4) To synthesize proteins
Fill in the blank: The enzyme __________ is used to synthesize DNA from an RNA template.
Fill in the blank: The first genetically engineered product approved for human use was __________.
Fill in the blank: The process of identifying and selecting cells that have incorporated recombinant DNA is called __________.
Fill in the blank: The process of introducing foreign DNA into a host cell is called __________.
True or False: All restriction enzymes cut DNA at the same sequence.
True or False: DNA ligase is used to cut DNA at specific sites.
True or False: Genetic engineering can be used to create organisms with new traits.
True or False: Genetic engineering can only be performed in bacteria.
