Principles of Genetic Engineering
Biology ⇒ Biotechnology and Applications
Principles of Genetic Engineering starts at 9 and continues till grade 12.
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Describe the difference between blunt ends and sticky ends produced by restriction enzymes.
Describe the function of a cloning vector in genetic engineering.
Describe the function of a reporter gene in genetic engineering experiments.
Describe the principle of gel electrophoresis in genetic engineering.
Explain the importance of host cells in genetic engineering.
Explain the role of restriction endonucleases in genetic engineering.
Explain the term 'recombinant DNA'.
Explain why antibiotic resistance genes are used as selectable markers in genetic engineering.
Which enzyme is used to synthesize complementary DNA (cDNA) from mRNA?
(1) DNA polymerase
(2) RNA polymerase
(3) Reverse transcriptase
(4) Ligase
Which of the following is a common host organism used in genetic engineering?
(1) Escherichia coli
(2) Saccharomyces cerevisiae
(3) Agrobacterium tumefaciens
(4) All of the above
Which of the following is a feature of plasmid vectors that makes them useful in genetic engineering?
(1) Ability to self-replicate
(2) Presence of selectable marker genes
(3) Small size
(4) All of the above
Which of the following is a limitation of genetic engineering?
(1) Ethical concerns
(2) Unintended effects on ecosystems
(3) High cost
(4) All of the above
Fill in the blank: The enzyme __________ is used to cut DNA at specific sequences.
Fill in the blank: The enzyme used to join DNA fragments together is called __________.
Fill in the blank: The process of identifying and selecting transformed cells is called __________.
Fill in the blank: The process of introducing recombinant DNA into a host cell is called __________.
True or False: A selectable marker helps identify cells that have taken up the recombinant DNA.
True or False: DNA ligase is required to seal the nicks in the sugar-phosphate backbone of DNA during recombinant DNA formation.
True or False: Restriction enzymes are also known as molecular scissors.
True or False: The polymerase chain reaction (PCR) is used to amplify DNA segments.
