Principles of Genetic Engineering
Biology ⇒ Biotechnology and Applications
Principles of Genetic Engineering starts at 9 and continues till grade 12.
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A researcher uses a restriction enzyme to cut a plasmid and a gene of interest. What must be true about the ends of the DNA fragments for successful ligation?
A scientist wants to clone a gene into a plasmid vector. Which enzyme should be used to join the gene and the plasmid?
Describe the difference between sticky ends and blunt ends produced by restriction enzymes.
Describe the function of restriction endonucleases in genetic engineering.
Describe the importance of palindromic sequences in genetic engineering.
Describe the process of transformation in genetic engineering.
A researcher uses a restriction enzyme to cut a plasmid and a gene of interest. What must be true about the ends of the DNA fragments for successful ligation?
A scientist wants to clone a gene into a plasmid vector. Which enzyme should be used to join the gene and the plasmid?
Which enzyme is used to synthesize complementary DNA (cDNA) from mRNA?
(1) DNA polymerase
(2) RNA polymerase
(3) Reverse transcriptase
(4) Ligase
Which of the following is a commonly used host cell in genetic engineering?
(1) E. coli
(2) Human red blood cell
(3) Platelet
(4) Neuron
Which of the following is a feature of plasmid vectors that makes them useful in genetic engineering?
(1) Ability to replicate independently
(2) Large size
(3) Inability to carry foreign DNA
(4) Lack of selectable markers
Which of the following is a method for introducing recombinant DNA into plant cells?
(1) Electroporation
(2) Microinjection
(3) Gene gun
(4) All of the above
Fill in the blank: The enzyme __________ is used in PCR to withstand high temperatures during DNA denaturation.
Fill in the blank: The enzyme __________ is used to cut DNA at specific sequences.
Fill in the blank: The enzyme used to join DNA fragments together is called __________.
Fill in the blank: The process of introducing DNA into animal cells using a fine glass micropipette is called __________.
True or False: All restriction enzymes produce sticky ends when they cut DNA.
True or False: Blunt ends are easier to ligate than sticky ends.
True or False: Recombinant DNA technology can be used to produce insulin for diabetic patients.
True or False: Restriction enzymes are also known as molecular scissors.
