Recombinant DNA Technology
Biology ⇒ Biotechnology and Applications
Recombinant DNA Technology starts at 9 and continues till grade 12.
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Describe one ethical concern related to recombinant DNA technology.
Describe one way recombinant DNA technology has improved agriculture.
Describe the difference between sticky ends and blunt ends in DNA fragments.
Describe the role of plasmids in recombinant DNA technology.
Explain the term 'transgenic organism'.
Explain the term 'vector' in the context of recombinant DNA technology.
Explain why antibiotic resistance genes are used in plasmid vectors.
Explain why restriction enzymes are important in recombinant DNA technology.
Which enzyme is responsible for cutting DNA at specific sequences?
(1) DNA polymerase
(2) Restriction enzyme
(3) RNA polymerase
(4) Ligase
Which of the following is a common host cell used in recombinant DNA experiments?
(1) E. coli
(2) Yeast
(3) Plant cells
(4) All of the above
Which of the following is a correct sequence of steps in recombinant DNA technology?
(1) Isolation, Ligation, Transformation, Selection
(2) Ligation, Isolation, Transformation, Selection
(3) Selection, Transformation, Ligation, Isolation
(4) Transformation, Ligation, Isolation, Selection
Which of the following is a limitation of recombinant DNA technology?
(1) High specificity
(2) Ethical concerns
(3) Production of medicines
(4) Improved crops
Fill in the blank: The _______ is the region on a plasmid where foreign DNA can be inserted.
Fill in the blank: The _______ technique is used to separate DNA fragments by size.
Fill in the blank: The enzyme _______ is used to synthesize DNA from an RNA template in some recombinant DNA procedures.
Fill in the blank: The first step in recombinant DNA technology is usually the _______ of the desired gene.
True or False: All restriction enzymes cut DNA to produce sticky ends.
True or False: DNA ligase is used to cut DNA at specific sites.
True or False: Recombinant DNA technology can be used to produce enzymes for industrial use.
True or False: Recombinant DNA technology can be used to produce hormones such as growth hormone.
