Recombinant DNA Technology
Biology ⇒ Biotechnology and Applications
Recombinant DNA Technology starts at 9 and continues till grade 12.
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See sample questions for grade 11
Describe one ethical issue related to the use of recombinant DNA technology.
Describe the difference between a genomic library and a cDNA library.
Describe the function of a multiple cloning site (MCS) in a vector.
Describe the role of Agrobacterium tumefaciens in plant genetic engineering.
Explain the difference between sticky ends and blunt ends produced by restriction enzymes.
A scientist wants to clone a gene into a plasmid vector. Which enzyme should she use to join the gene and the plasmid?
(1) DNA polymerase
(2) DNA ligase
(3) RNA polymerase
(4) Restriction endonuclease
A biotechnology company is developing a genetically modified bacterium to produce a human enzyme. What two features must the expression vector contain to ensure both selection and high-level expression of the enzyme?
A scientist is attempting to clone a gene that is 10,000 base pairs long. Which type of vector would be most suitable for cloning such a large DNA fragment?
(1) Plasmid
(2) Bacterial Artificial Chromosome (BAC)
(3) Yeast Artificial Chromosome (YAC)
(4) Cosmid
A scientist wants to clone a gene into a plasmid vector. Which enzyme should she use to join the gene and the plasmid?
(1) DNA polymerase
(2) DNA ligase
(3) RNA polymerase
(4) Restriction endonuclease
Which enzyme is used to cut DNA at specific sites?
(1) DNA polymerase
(2) Restriction endonuclease
(3) RNA polymerase
(4) Ligase
Which of the following is a commonly used bacterial plasmid vector?
(1) pBR322
(2) Ti plasmid
(3) Lambda phage
(4) BAC
Which of the following is a major application of recombinant DNA technology in medicine?
(1) Production of antibiotics
(2) Production of synthetic fibers
(3) Production of human insulin
(4) Water purification
Fill in the blank: The enzyme __________ is used to synthesize complementary DNA (cDNA) from mRNA.
Fill in the blank: The enzyme __________ is used to synthesize DNA from a DNA template during PCR.
Fill in the blank: The enzyme used to join DNA fragments together is called __________.
Fill in the blank: The first recombinant DNA molecule was constructed by Paul Berg in the year __________.
True or False: DNA ligase can join both sticky and blunt ends of DNA fragments.
True or False: Plasmids are circular, double-stranded DNA molecules found in bacteria.
True or False: Recombinant DNA technology can be used to create genetically modified crops with improved traits.
True or False: Recombinant DNA technology can be used to produce vaccines such as the hepatitis B vaccine.
