Recombinant DNA Technology
Biology ⇒ Biotechnology and Applications
Recombinant DNA Technology starts at 9 and continues till grade 12.
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Describe one application of recombinant DNA technology in medicine.
Describe one ethical concern related to recombinant DNA technology.
Describe the process of gene cloning using a plasmid vector.
Describe the role of a vector in recombinant DNA technology.
Explain the difference between blunt ends and sticky ends in DNA fragments.
Explain the term 'sticky ends' in the context of restriction enzyme action.
Explain why antibiotic resistance genes are used in plasmid vectors.
Name one commonly used restriction enzyme in recombinant DNA technology.
Which of the following is a commonly used host organism in recombinant DNA technology?
(1) Escherichia coli
(2) Saccharomyces cerevisiae
(3) Agrobacterium tumefaciens
(4) All of the above
Which of the following is a feature of a plasmid vector?
(1) Antibiotic resistance gene
(2) Origin of replication
(3) Multiple cloning site
(4) All of the above
Which of the following is a method for screening recombinant bacteria?
(1) Blue-white screening
(2) Gram staining
(3) Southern blotting
(4) PCR
Which of the following is a palindromic sequence?
(1) GAATTC
(2) GATTAC
(3) ATCGTA
(4) CGTACG
Fill in the blank: The enzyme __________ is used to synthesize complementary DNA (cDNA) from mRNA.
Fill in the blank: The enzyme used to cut DNA at specific sites is called __________.
Fill in the blank: The enzyme used to join DNA fragments together is called __________.
Fill in the blank: The process of introducing DNA into animal cells using electric pulses is called __________.
True or False: DNA ligase forms phosphodiester bonds between adjacent nucleotides.
True or False: Recombinant DNA technology can be used to produce enzymes for industrial use.
True or False: Recombinant DNA technology can be used to produce vaccines against infectious diseases.
True or False: Restriction enzymes cut DNA at specific recognition sequences.
