Recombinant DNA Technology
Biology ⇒ Biotechnology and Applications
Recombinant DNA Technology starts at 9 and continues till grade 12.
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See sample questions for grade 9
Describe one benefit and one risk of using genetically modified crops.
Describe one ethical concern related to recombinant DNA technology.
Describe the function of restriction enzymes in recombinant DNA technology.
Explain the term 'genetically modified organism' (GMO) in the context of recombinant DNA technology.
Explain why bacteria are often used in recombinant DNA experiments.
Explain why recombinant DNA technology is important in agriculture.
Explain why restriction enzymes are called 'molecular scissors'.
A researcher is using recombinant DNA technology to create bacteria that can break down oil spills. What is one challenge the researcher might face when releasing these bacteria into the environment?
Which enzyme is responsible for joining the ends of DNA fragments?
(1) DNA polymerase
(2) DNA ligase
(3) Restriction enzyme
(4) RNA polymerase
Which of the following best describes a vector in recombinant DNA technology?
(1) A virus that causes disease
(2) A molecule used to transfer foreign DNA into a host cell
(3) An enzyme that cuts DNA
(4) A type of cell membrane
Which of the following is a correct sequence of steps in recombinant DNA technology?
(1) Isolation of gene → Insertion into vector → Introduction into host
(2) Insertion into vector → Isolation of gene → Introduction into host
(3) Introduction into host → Isolation of gene → Insertion into vector
(4) Isolation of gene → Introduction into host → Insertion into vector
Which of the following is a reason for using antibiotic resistance genes in plasmids?
(1) To make bacteria glow
(2) To select for bacteria that have taken up the plasmid
(3) To increase the size of the plasmid
(4) To destroy the host cell
Fill in the blank: The first step in recombinant DNA technology is usually the __________ of the desired gene.
Fill in the blank: The process of introducing recombinant DNA into a host cell is called __________.
Fill in the blank: The process of making an exact copy of a gene using recombinant DNA technology is called gene __________.
Fill in the blank: The process of making multiple copies of a gene using recombinant DNA technology is called __________.
True or False: DNA ligase is used to join DNA fragments together.
True or False: Plasmids are commonly used as vectors in recombinant DNA technology.
True or False: Recombinant DNA technology can be used to create animals with new traits.
True or False: Recombinant DNA technology can be used to produce vaccines.
