Cellular Microscopy Techniques
Biology ⇒ Cell Biology
Cellular Microscopy Techniques starts at 8 and continues till grade 12.
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Describe how differential interference contrast (DIC) microscopy differs from phase-contrast microscopy.
Describe one reason why electron microscopes have higher resolving power than light microscopes.
Describe the main difference between transmission electron microscopy (TEM) and scanning electron microscopy (SEM).
Describe the main limitation of using high magnification in light microscopy.
Explain why living cells cannot be observed with a transmission electron microscope.
Explain why samples must be placed in a vacuum for electron microscopy.
Explain why staining is often necessary in light microscopy.
Name one advantage and one limitation of using fluorescence microscopy.
Which microscopy technique is best suited for observing live, unstained cells? (1) Bright-field microscopy (2) Phase-contrast microscopy (3) Scanning electron microscopy (4) Transmission electron microscopy
Which microscopy technique uses antibodies tagged with fluorescent dyes to detect specific proteins in cells? (1) Phase-contrast microscopy (2) Immunofluorescence microscopy (3) Scanning electron microscopy (4) Bright-field microscopy
Which of the following best describes the function of the diaphragm in a light microscope? (1) Adjusts magnification (2) Focuses the image (3) Regulates the amount of light passing through the specimen (4) Moves the stage
Which of the following best describes the principle of phase-contrast microscopy? (1) Uses electron beams (2) Enhances contrast by amplifying differences in refractive index (3) Uses fluorescent dyes (4) Requires staining
Fill in the blank: In confocal microscopy, a ________ is used to focus light on a single plane within the specimen.
Fill in the blank: In fluorescence microscopy, the fluorescent dye is excited by ________ light and emits ________ light.
Fill in the blank: The ________ is the flat platform where the slide is placed in a light microscope.
Fill in the blank: The ________ is the part of the microscope that you look through to view the specimen.
True or False: Electron microscopes can be used to observe living cells.
True or False: The eyepiece of a light microscope usually provides additional magnification.
True or False: The resolving power of a microscope is determined by the wavelength of the illumination used.
True or False: The scanning electron microscope can provide color images of cell surfaces.
