Laboratory Techniques in Microbiology
Biology ⇒ Microorganisms and Microbiology
Laboratory Techniques in Microbiology starts at 9 and continues till grade 12.
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See sample questions for grade 11
A student is preparing a Gram stain and forgets to add the decolorizer. What will be the likely result?
A student observes that after Gram staining, some bacteria appear pink while others appear purple. What does this indicate about the bacteria?
Describe the main difference between selective and differential media.
Describe the main purpose of using a streak plate method in microbiology.
A student is preparing a Gram stain and forgets to add the decolorizer. What will be the likely result?
A student observes that after Gram staining, some bacteria appear pink while others appear purple. What does this indicate about the bacteria?
A laboratory technician is using a spectrophotometer to measure the growth of a bacterial culture. Explain how the optical density (OD) reading relates to the number of bacteria present and discuss one limitation of this method.
A student is given an unknown bacterial sample and asked to determine if it produces endospores. Describe the staining technique that should be used and the expected results if endospores are present.
Which biosafety level (BSL) is required for handling highly infectious agents such as Ebola virus?
(1) BSL-1
(2) BSL-2
(3) BSL-3
(4) BSL-4
Which of the following is a differential staining technique?
(1) Simple stain
(2) Gram stain
(3) Negative stain
(4) Endospore stain
Which of the following is NOT a component of nutrient agar?
(1) Peptone
(2) Agar
(3) Sodium chloride
(4) Ethanol
Which of the following is NOT a reason for using a laminar flow hood in microbiology?
(1) To protect the user from pathogens
(2) To prevent contamination of samples
(3) To sterilize glassware
(4) To maintain a sterile work environment
Fill in the blank: The ________ is used to maintain a constant temperature for the growth of microorganisms.
Fill in the blank: The ________ method is commonly used to test the effectiveness of antibiotics against bacteria on an agar plate.
Fill in the blank: The ________ method is used to determine the minimum inhibitory concentration (MIC) of an antibiotic.
Fill in the blank: The ________ technique is used to estimate the number of viable microorganisms in a sample by counting colonies.
True or False: The autoclave uses dry heat to sterilize laboratory equipment.
True or False: The catalase test is performed by adding hydrogen peroxide to a bacterial culture and observing for bubble formation.
True or False: The Gram staining technique differentiates bacteria based on the composition of their cell walls.
True or False: The pour plate method can be used to estimate the number of viable bacteria in a sample.
