Laboratory Techniques in Microbiology
Biology ⇒ Microorganisms and Microbiology
Laboratory Techniques in Microbiology starts at 9 and continues till grade 12.
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See sample questions for grade 12
A student is using a spectrophotometer to measure the turbidity of a bacterial culture. What is being estimated by this method?
A student observes red colonies on MacConkey agar after incubating a sample. What does this indicate about the bacteria?
A student prepares a smear that is too thick. What problem might this cause during microscopic examination?
Describe how you would prepare a bacterial smear from a solid culture for staining.
A student is asked to calculate the number of colony-forming units (CFUs) per mL in a sample. If 0.1 mL of a 10-4 dilution yields 50 colonies, what is the CFU/mL in the original sample?
A student is using a spectrophotometer to measure the turbidity of a bacterial culture. What is being estimated by this method?
A student observes red colonies on MacConkey agar after incubating a sample. What does this indicate about the bacteria?
A student prepares a smear that is too thick. What problem might this cause during microscopic examination?
Which of the following best describes the function of an autoclave?
(1) To dry glassware
(2) To sterilize media and equipment using high-pressure steam
(3) To incubate cultures
(4) To filter solutions
Which of the following is a differential staining technique?
(1) Simple stain
(2) Gram stain
(3) Negative stain
(4) Capsule stain
Which of the following is a reason for using a water bath during the melting of agar?
(1) To prevent overheating and degradation of agar
(2) To sterilize the agar
(3) To cool the agar rapidly
(4) To increase the pH of the agar
Which of the following is a selective medium for Gram-negative bacteria?
(1) MacConkey agar
(2) Blood agar
(3) Sabouraud agar
(4) Nutrient agar
In the Gram staining procedure, the primary stain used is __________.
The clear area around an antibiotic disc on an agar plate is called the __________ of inhibition.
The process of killing all forms of microbial life, including spores, is called __________.
The process of reducing the number of pathogenic microorganisms to a safe level is called __________.
True or False: The catalase test is used to differentiate between Staphylococcus and Streptococcus species.
True or False: The Gram staining technique differentiates bacteria based on the composition of their cell wall.
True or False: The pour plate method can be used to estimate the number of viable microorganisms in a sample.
True or False: The spread plate method can be used to obtain isolated colonies from a mixed culture.
