Microscopy in Microbiology
Biology ⇒ Microorganisms and Microbiology
Microscopy in Microbiology starts at 8 and continues till grade 12.
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See sample questions for grade 11
Describe one advantage and one limitation of using electron microscopy in microbiology.
Describe the main advantage of using fluorescence microscopy in microbiology.
Describe the principle of dark-field microscopy.
Describe the purpose of staining in light microscopy.
Explain the main difference between a scanning electron microscope (SEM) and a transmission electron microscope (TEM).
Explain the main reason why staining is not required for phase-contrast microscopy.
A student is using a microscope with a 10x ocular lens and a 100x oil immersion objective lens. What is the total magnification?
A student observes a bacterial cell under a microscope using a 10x ocular lens and a 40x objective lens. What is the total magnification?
A microbiologist wants to observe the motility of live bacteria. Which type of microscopy should they use?
(1) Bright-field
(2) Phase-contrast
(3) Scanning electron
(4) Transmission electron
Which microscope would be most suitable for viewing living, unstained microorganisms?
(1) Bright-field microscope
(2) Phase-contrast microscope
(3) Scanning electron microscope
(4) Transmission electron microscope
Which of the following best describes the function of the objective lens in a compound microscope?
(1) To focus light onto the specimen
(2) To magnify the image of the specimen
(3) To support the slide
(4) To adjust the intensity of light
Which of the following is a correct statement about phase-contrast microscopy?
(1) It requires staining of specimens.
(2) It enhances contrast in transparent specimens without staining.
(3) It is used only for dead cells.
(4) It uses electron beams.
Fill in the blank: The lens closest to the eye in a compound microscope is called the ________ lens.
Fill in the blank: The maximum resolving power of a typical light microscope is about ________ micrometers.
Fill in the blank: The process of attaching a specimen to a slide by heating is called ________.
Fill in the blank: The smallest distance between two points that can still be distinguished as separate is called ________.
True or False: Confocal microscopy uses lasers to produce sharp, three-dimensional images of specimens.
True or False: Fluorescence microscopy can be used to detect specific proteins in cells using fluorescently labeled antibodies.
True or False: Oil immersion is used with the 100x objective lens to increase the resolving power of a light microscope.
True or False: The coarse adjustment knob is used for fine focusing at high magnification.
