Microscopy in Microbiology
Biology ⇒ Microorganisms and Microbiology
Microscopy in Microbiology starts at 8 and continues till grade 12.
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Describe one advantage and one limitation of using fluorescence microscopy in microbiology.
Describe the main difference between simple and compound microscopes.
Describe the principle of dark-field microscopy.
Explain the main difference between a scanning electron microscope (SEM) and a transmission electron microscope (TEM).
A microbiologist observes a specimen using a 100x oil immersion objective and a 10x ocular lens. What is the total magnification?
A microbiologist wants to observe the detailed internal structure of a virus. Which microscope should they use?
(1) Light microscope
(2) Scanning electron microscope
(3) Transmission electron microscope
(4) Dissecting microscope
A student observes a bacterial cell under a microscope using a 10x ocular lens and a 40x objective lens. What is the total magnification?
Which type of microscopy is most suitable for visualizing the arrangement of flagella on bacterial cells?
(1) Bright-field microscopy
(2) Dark-field microscopy
(3) Scanning electron microscopy
(4) Phase-contrast microscopy
A microbiologist wants to observe the detailed internal structure of a virus. Which microscope should they use?
(1) Light microscope
(2) Scanning electron microscope
(3) Transmission electron microscope
(4) Dissecting microscope
Which of the following best describes the working principle of fluorescence microscopy?
(1) Uses visible light to illuminate specimens
(2) Uses ultraviolet light to excite fluorescent dyes attached to the specimen
(3) Uses electron beams to scan the surface
(4) Uses sound waves to create images
Which of the following is a limitation of electron microscopy?
(1) Low magnification
(2) Inability to view living specimens
(3) Poor resolution
(4) Inability to view viruses
Which of the following is NOT a component of a compound light microscope?
(1) Objective lens
(2) Ocular lens
(3) Electron gun
(4) Stage
The ability of a microscope to increase the apparent size of an object is called __________.
The minimum distance at which two points can be distinguished as separate in a microscope is called __________.
The numerical aperture (NA) of a lens affects the __________ of a microscope.
The part of the microscope that regulates the amount of light passing through the specimen is called the __________.
True or False: Confocal microscopy can be used to construct three-dimensional images of microbial communities.
True or False: Electron microscopes can be used to observe living microorganisms.
True or False: Phase-contrast microscopy enhances the contrast of transparent and colorless specimens without staining.
True or False: The condenser lens focuses light onto the specimen in a compound light microscope.
