Sterilization and Aseptic Techniques
Biology ⇒ Microorganisms and Microbiology
Sterilization and Aseptic Techniques starts at 10 and continues till grade 12.
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Describe how you would sterilize a glass petri dish in the laboratory.
Describe one reason why aseptic techniques are important in hospitals.
Describe one situation where dry heat sterilization is preferred over moist heat sterilization.
Describe the main difference between sterilization and disinfection.
Describe the principle behind filtration as a method of sterilization.
Explain the importance of using sterile gloves during surgical procedures.
Explain why aseptic techniques are important in microbiology laboratories.
Explain why hands should be washed before and after handling microbial cultures.
Autoclaving uses steam under pressure. What is the standard temperature and pressure used in autoclaving?
(1) 100°C, 1 atm
(2) 121°C, 15 psi
(3) 140°C, 20 psi
(4) 80°C, 5 psi
Which of the following is a chemical method of sterilization?
(1) Ethylene oxide gas
(2) Boiling
(3) UV radiation
(4) Filtration
Which of the following is a limitation of using UV radiation for sterilization?
(1) It is expensive
(2) It cannot penetrate surfaces
(3) It produces toxic gases
(4) It is too slow
Which of the following is NOT a correct step in aseptic technique?
(1) Sterilizing equipment before use
(2) Working near an open flame
(3) Touching sterile surfaces with bare hands
(4) Minimizing talking during procedures
Fill in the blank: The process of burning materials to ashes to destroy microorganisms is called __________.
Fill in the blank: The process of heating milk to 72°C for 15 seconds to kill pathogens is called __________.
Fill in the blank: The process of using high temperatures to kill all microorganisms on surgical instruments is called __________.
Fill in the blank: The use of __________ is essential to prevent contamination when transferring microorganisms.
True or False: Alcohol is used to sterilize skin before injections.
True or False: Flaming the mouth of a test tube before and after use is an example of an aseptic technique.
True or False: Incineration is a method used to sterilize disposable medical waste.
True or False: Pasteurization is a method of sterilization.
