Sterilization and Aseptic Techniques
Biology ⇒ Microorganisms and Microbiology
Sterilization and Aseptic Techniques starts at 10 and continues till grade 12.
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A laboratory technician needs to sterilize a solution of vitamins that are heat-labile. Which method should they use?
A researcher needs to sterilize a solution containing antibiotics that degrade at high temperatures. Which method should be used and why?
A student is preparing to inoculate a sterile agar plate with bacteria. List three aseptic techniques they should follow to prevent contamination.
Describe the difference between antiseptics and disinfectants.
A laboratory technician needs to sterilize a solution of vitamins that are heat-labile. Which method should they use?
A researcher needs to sterilize a solution containing antibiotics that degrade at high temperatures. Which method should be used and why?
A student is preparing to inoculate a sterile agar plate with bacteria. List three aseptic techniques they should follow to prevent contamination.
A laboratory is testing the effectiveness of a new sterilization method. After treatment, samples are incubated in nutrient broth, and turbidity is observed in some tubes. What does the presence of turbidity indicate, and what should be the next step in evaluating the sterilization method?
Which chemical is commonly used for sterilizing plastic syringes?
(1) Ethylene oxide
(2) Alcohol
(3) Phenol
(4) Chlorine
Which of the following is a correct statement about aseptic technique?
(1) It is only used in hospitals
(2) It prevents contamination of cultures and the environment
(3) It is not necessary when working with non-pathogenic organisms
(4) It involves only hand washing
Which of the following is a limitation of chemical sterilization?
(1) It is always effective against spores
(2) It can leave toxic residues
(3) It is suitable for all materials
(4) It is faster than autoclaving
Which of the following is a limitation of UV radiation as a sterilization method?
(1) It is expensive
(2) It cannot penetrate surfaces
(3) It is toxic to humans
(4) It is slow
Fill in the blank: The minimum time required to sterilize glassware in a hot air oven at 160°C is __________ minutes.
Fill in the blank: The process of incineration is mainly used for the disposal of __________ materials.
Fill in the blank: The process of killing or removing all microorganisms, including spores, from an object is called __________.
Fill in the blank: The process of using high pressure and steam to sterilize equipment is called __________.
Is it necessary to use aseptic techniques when transferring bacteria from one culture medium to another? (Yes/No)
True or False: All sterilization methods are suitable for all types of materials.
True or False: Boiling water for 10 minutes is sufficient to sterilize surgical instruments.
True or False: Ethylene oxide gas can be used to sterilize heat-sensitive medical equipment.
