Sterilization and Aseptic Techniques
Biology ⇒ Microorganisms and Microbiology
Sterilization and Aseptic Techniques starts at 10 and continues till grade 12.
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A laboratory technician needs to sterilize glass petri dishes. Which method should be used?
A researcher needs to sterilize a solution containing heat-labile vitamins. Which method should be used and why?
Describe the difference between disinfection and sterilization.
Describe the importance of using sterile gloves and gowns during medical procedures.
Describe the principle of filtration as a sterilization method.
Describe the role of laminar airflow cabinets in aseptic techniques.
A laboratory technician needs to sterilize glass petri dishes. Which method should be used?
A researcher needs to sterilize a solution containing heat-labile vitamins. Which method should be used and why?
Which of the following is a chemical sterilizing agent?
(1) Ultraviolet light
(2) Ethylene oxide
(3) Boiling water
(4) Filtration
Which of the following is a correct statement about aseptic techniques?
(1) They are only used in hospitals
(2) They prevent contamination of cultures and the environment
(3) They are not necessary in microbiology labs
(4) They only involve hand washing
Which of the following is a limitation of using UV radiation for sterilization?
(1) It penetrates deeply into solids
(2) It can only sterilize surfaces
(3) It is effective against spores
(4) It is suitable for liquids
Which of the following is NOT a limitation of chemical sterilization?
(1) Toxicity
(2) Corrosiveness
(3) Inability to sterilize heat-sensitive items
(4) Need for aeration after use
Fill in the blank: ________ is the process of reducing the number of microorganisms to a safe level, but not necessarily eliminating all of them.
Fill in the blank: The ________ test is used as a biological indicator to check the effectiveness of autoclave sterilization.
Fill in the blank: The minimum time required for autoclaving at 121°C is ______ minutes.
Fill in the blank: The process of burning materials to ashes to achieve sterilization is called __________.
True or False: Alcohol is an effective sterilizing agent for surgical instruments.
True or False: All sterilization methods are suitable for all types of materials.
True or False: Flaming the mouth of a test tube before and after use is an example of an aseptic technique.
True or False: Sterilization by dry heat requires higher temperatures and longer exposure times than moist heat sterilization.
