Plant Tissue Culture
Biology ⇒ Plant Biology
Plant Tissue Culture starts at 10 and continues till grade 12.
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See sample questions for grade 12
Define 'callus' in the context of plant tissue culture.
Describe the difference between organogenesis and somatic embryogenesis in plant tissue culture.
Describe the process of protoplast culture.
Describe the role of carbon source in plant tissue culture media.
Explain the term 'totipotency' in plant cells.
Explain why aseptic conditions are critical in plant tissue culture.
A researcher is attempting to culture a rare orchid species but observes that the explants consistently fail to develop roots, even though shoots are forming. Suggest two possible reasons for this failure and propose solutions based on plant tissue culture principles.
A student is preparing a plant tissue culture medium and accidentally omits the addition of a carbon source. Predict and explain the likely outcome for the cultured explants.
Which of the following is a direct application of plant tissue culture in agriculture?
(1) Production of transgenic plants
(2) Soil testing
(3) Crop rotation
(4) Manual pollination
Which of the following is a disadvantage of plant tissue culture?
(1) Production of disease-free plants
(2) High initial cost
(3) Rapid multiplication
(4) Conservation of rare species
Which of the following is a limitation of micropropagation?
(1) Uniformity of plants
(2) High multiplication rate
(3) Somaclonal variation
(4) Disease elimination
Which of the following is a major advantage of plant tissue culture?
(1) Slow propagation
(2) Disease-free plants
(3) High cost
(4) Limited genetic diversity
Fill in the blank: The first step in plant tissue culture is the selection and preparation of the _______.
Fill in the blank: The nutrient medium used in plant tissue culture must contain _______ as a carbon source.
Fill in the blank: The process of fusing two protoplasts from different species is called _______.
Fill in the blank: The process of growing plant cells in a liquid medium with constant agitation is called _______ culture.
True or False: All plant species can be cultured successfully using the same tissue culture protocol.
True or False: Cytokinins promote shoot formation in plant tissue culture.
True or False: Explants must be washed thoroughly with sterile water after surface sterilization before being placed on culture media.
True or False: Plant tissue culture can be used to produce virus-free plants.
