Chromatography
Chemistry ⇒ Analytical Chemistry
Chromatography starts at 12 and continues till grade 12.
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See sample questions for grade 12
A chromatogram shows three peaks at retention times of 2 min, 5 min, and 8 min. What does this indicate about the sample?
A chromatographic separation resulted in overlapping peaks. Suggest one way to improve the resolution.
A student uses TLC to separate a mixture and observes that two spots have the same Rf value. What could this indicate?
Define retention time in chromatography.
A chromatogram shows three peaks at retention times of 2 min, 5 min, and 8 min. What does this indicate about the sample?
A chromatographic separation resulted in overlapping peaks. Suggest one way to improve the resolution.
A student performed paper chromatography and found that the blue dye traveled 5 cm while the solvent front traveled 10 cm. What is the Rf value of the blue dye?
A student uses TLC to separate a mixture and observes that two spots have the same Rf value. What could this indicate?
Which of the following best describes the use of a gradient elution in HPLC? (1) Using a constant mobile phase composition (2) Gradually changing the composition of the mobile phase during the run (3) Increasing the temperature during the run (4) Decreasing the flow rate during the run
Which of the following is a common application of gas chromatography? (1) Separation of proteins (2) Analysis of essential oils (3) Separation of DNA fragments (4) Detection of metal ions
Which of the following is a key advantage of using HPLC over TLC? (1) Lower cost (2) Higher sensitivity and precision (3) Simpler equipment (4) No need for detectors
Which of the following is a limitation of paper chromatography? (1) Cannot separate colored compounds (2) Not suitable for volatile substances (3) Requires expensive equipment (4) Cannot be used for amino acids
In affinity chromatography, the stationary phase is designed to bind specifically to the _______ of interest.
In chromatography, the process of removing adsorbed substances from the stationary phase is called _______.
In ion-exchange chromatography, separation is based on the _______ of the molecules.
In paper chromatography, the solvent used as the mobile phase is called the _______.
True or False: Chromatography can be used for both qualitative and quantitative analysis.
True or False: In chromatography, the stationary phase is always a solid.
True or False: In column chromatography, larger particles elute faster than smaller particles in size-exclusion chromatography.
True or False: In gas chromatography, the mobile phase is a liquid.
